These products are all based on our proven and standardized silica gels for production
chromatography. Starting material is a silica gel with a nominal pore diameter of 60Å
that has been effectively used over many decades.
The silanized silica gel (Cat. No. 7719) is a material that has been slightly deactivated by
dimethylsilyl groups and which has a high specificity for the separation of very polar, acid
or strongly lipophilic substances. This material is comparable in polarity with RP-2 material
and can be placed between an RP-8 and a cyano-phase.
Silica gel 60 RP-18 (40-63µm) (Cat. No. 10167) is a stationary phase modified with C18
groups and is ideal for the purification of compounds of molecular weight lower than 1,000 Daltons
in semi-preparative and preparative scale. |
 |
The Ideal Reversed Phase Material for Production Scale
| |
An alternative material to non-modified silica gel because non-toxic solvents in the RP-mode are
used.
For the economic purification of products where conventional RP-phases were too expensive.
High loading capacity due to its high specific surface area of the base silica gel (60Å
pore diameter, approximately 500m2/g); therefore column dimensions/amount of sorbent can
be reduced or the sample volume increased.
High pressure stability under extreme production conditions for long-term column usage. |
| Specifications: Silica Gel 60 RP-18 (40-63µm) for Preparative HPLC |
| Particle Size |
90% between 40 and 64µm |
| Elementary Analysis |
15-20% C |
| Elutable Fraction |
<0.2% |
| Mean Pore Size |
60Å |
Selectivity Test
k-value (Benzene) 7.2-10.8
alpha = Acetophenone/Phenol 1.7-2.3
alpha = Acetophenone/Cinnamic acid 1.1-1.5
alpha =Naphthol/Phenol 3.6-4.6
Comparison of Selectivity Profile
| |
LiChroprep® RP18 (40-63µm) |
Silica Gel 60 RP-18 (40-63µm) |
| k-value (Benzene) |
7.65-10.35 |
7.2-10.8 |
| alpha=(1)Acetophenon/Phenol |
1.9-2.1 |
1.7-2.3 |
| alpha=(2)Acetophenon/Cinnamic Acid |
1.23-1.36 |
1.1-1.5 |
| alpha=(3)Naphthol/Phenol |
3.8-4.2 |
3.6-4.6 |
Comparison of Different Stationary Phases show the following values under identical
chromatgraphic conditions:
| Stationary Phase |
alpha (1) |
alpha (2) |
alpha (2) |
k (benzene) |
| LiChrospher® RP-18 (12µm) |
2.1 |
1.36 |
3.8 |
10.3 |
| LiChrosorb® RP-18 (7µm) |
1.98 |
1.28 |
3.8 |
9.8 |
| LiChroprep® RP-18 (40-63µm) |
2.0 |
1.28 |
3.8 |
10.2 |
| Silica Gel 60 RP-18 (40-63µm) |
2.3 |
1.50 |
3.87 |
10.6 |
|
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