Anaerocult® C-mini

EMD Cat. No. 13699
1 x 25


For producing an anaerobic environment in an anaerobic jar (Anaerocult® A), for the anaerobic incubation in special bags in identification galleries or microtitre plates (Anaerocult® IS), or individual Petri dishes (Anaerocult® A mini or P), or for generating an O2-deficient, CO2-enriched atmosphere in an anaerobic jar (Anaerocult® C), or in special incubation bags for 1 or 2 Petri dishes (Anaerocult® C mini).

Typical Composition (g/liter) Literature                        


Mode of Action
 The Anaerocult® system are filter-paper bags of different sizes that are filled with an oxygen-binding reagent mixture which also produces carbon dioxide. Water is added to start the reaction which consists of the oxidation of finely divided iron; the reaction proceeds without a catalyst. Anaerocult® A, A mini, P and IS produce an anaerobic environment indicated by the test strip. Anaerotest®, Anaerocult® C and Anaerocult® C mini, however, produce an O2-enriched atmosphere which is required, for example, for the cultivation of Campylobacter and other microaerophilic organisms.
Experimental Procedure for Anaerocult® A
 Stack the inoculated Petri dishes in the plate basket and place in the Anaerobic jar. Moisten an Anerocult® A sachet with 35 ml water and place in the Anaerobic jar next to the plate basket. An Anaerotest® test-strip moistened with a drop of water should also be included. Seal the jar and incubate. Petri dishes with ventilation lifters should be used to improve gas exchange.
Experimental Procedure for Anaerocult® P
 Place the Anaerocult® P sachet in the special incubation container (foil bag) and moisten with 3 ml water. Immediately place the inoculated Petri dish in the incubation container; fix an Anaerotest® test-strip moistened with a drop of water to the lid of the Petri dish (reaction zone showing downwards). Seal the container with a conventional foil sealer or with Anaeroclip® and incubate in the incubator. Petri dishes with ventilation lifters should be used if possible.
Experimental Procedure for Anaerocult® A mini
 Place the Anaerocult® A mini sachet in the special incubation container (foil bag) and moisten with 8 ml water. Immediately place the inoculated Petri dishes (maximum 4) in the incubation container; it is best to include a moistened Anaerotest® test-strip. Seal the container with a conventional foil sealer or with Anaeroclip® and incubate in the incubator. Petri dishes with ventilation lifters should be used if possible.
Experimental Procedure for Anaerocult® C
 The procedure is the same as described for Anaerocult® A but with the following differences: the Anaerocult® sachet is moistened with 6 ml water and an Anaerotest® test-strip is not added.
Experimental Procedure for Anaerocult® C mini
 An Anaerocult® C mini bag is placed in the special incubation sachet and moistened with 3 ml of water. Then 1 or 2 inoculated Petri dishes are added. The incubation sachet is sealed with a commercially available foil sealer or with Anaeroclip® and placed in the incubator. An Anaerotest® test-strip is not added.
Typical Composition
 Kieselguhr
 Iron powder
 Citric acid
 Sodium carbonate
Literature

COSTIN, J.D., FISCHER, W., KAPPNER, M., SCHMIDT, W., u. SCHUCHMANN, H.: Kultivierung von anaeroben Mikroorganismen. Eine neue Methode zur Erzeugung eines anaeroben Milieus. - Forum Mikrobiol., 5; 246-248 (1982).
 COSTIN, J.D., KAPPNER, M., u. SCHMIDT, W.: Anaerocult® C - ein neues System für die Kultivierung von mikroaerophilen und capneischen Mikroorganismen. - Poster bei 1st European Congress of Clinical Microbiology, Bologna (1983).
 SCHULER, R.: Das Anaerocult®-Verfahren in der bakteriologischen Untersuchungstechnik. - Ärztl. Lab., 29; 359-361 (1983)

© 2002 Merck KGaA, Darmstadt, Germany